PRODUKTIVITAS BIOETANOL DARI KAYU SENGON (Paraserianthes
falcataria) DENGAN PERLAKUAN
ENZIMATIS
(BIOETHANOL
PRODUCTIVITY FROM SENGON WOOD (Paraserianthes falcataria) USING ENZIMATIC
TREATMENTS)
Muhammad Daud1,
Wasrin
Syafii2, Khaswar Syamsu3
1Fakultas Kehutanan,
Universitas Hasanuddin
2Departemen Hasil Hutan, IPB
3Departemen Teknologi
Industri Pertanian, IPB
ABSTRACT
This
study aims determine the best enzymatic treatments to produce bioethanol from
from sengon wood using simulataneous
saccharification and fermentation (SSF)
processes. Sengon wood (Paraserianthes falcataria) were pretreated using kraft process to
remove lignin content. Pulp were produced from kraft process were analize to
determine their chemical properties before treatments.Then, pulp treated using SSF processes. SSF
runs were performed in 500 ml fermentors using a total slurry 200 ml. The substrate and
nutrient media were autoclaved (121 oC and 20 minutes). The samples
diluted to 2,5% (w/v) of total slurry was used as substrate. The substrate was
added with two kinds of enzymatic treatments namely 4% and 8% of total slurry and then inoculated
with 10% (v/v) yeast Saccharomyces cereviciae (1,5
x 109 CFU/cc). The SSF experiments were run for 96 hours and the data
were investigated five time namely 0, 24, 48, 72 and 96 hours. The results
showed that total of sugar and reducing sugar tended to decrease with time of
inoculation whereas ethanol concentration increase significantly. The growth of
Saccharomyces cereviciae yeast tended to incease in initial inoculation and
decrease by the end of inoculation. The best method to hydrolyse
(saccharification) and fermentation on SSF processes were using cellulase 8% of
dry mass (DM) and 10% (v/v) of
Saccharomyces cereviciae and run for 96 hours which produced highest
bioethanol was 0,79% and produced yields 8,98% with productivity 2991,97
litre/ha/year.
Key
words: Bioethanol, enzimatic treatments,
productivity, sengon wood, cellulase
