PEMANFAATAN BATANG KELAPA SAWIT (Elaeis
guineensis Jacq.) MENJADI BIOETANOL DENGAN PERLAKUAN PENDAHULUAN
MENGGUNAKAN PROSES KRAFT
(UTILIZATION
OIL PALM (Elaeis guineensis Jacq.) STEM FOR BIOETHANOL PRODUCTION WITH
PRETREATMENT USING
KRAFT
PROCESS)
Muhammad Daud1,
Wasrin
Syafii1, Khaswar Syamsu2
1Departemen Hasil Hutan, IPB
2Departemen Teknologi
Industri Pertanian, IPB
ABSTRACT
This
study aims to determine the best pretreatment and time of inoculation to
produce bioethanol from oil palm (Elaeis
guineensis Jacq.) stem using simulataneous
saccharification and fermentation (SSF)
processes with Aspergillus
niger dan Saccharomyces cereviciae. The samples were chipped and then milled
to determine their chemical properties before treatments. The study was
conducted to determine the best pretreatment to produce the highest yield of
pulp and low of kappa number and lignin content
The treatments were conducted under three kinds of alkalinity (active
alkali) namely 16, 18 and 20% and also two kinds of sulfidity namely 20 and 25%
using kraft process. Then, pulp
treated using SSF processes. SSF runs were performed in 500 ml
fermentors using a total slurry 200 ml.
The substrate and nutrient media were autoclaved (121 oC and 20
minutes). The samples diluted to 2,5% (w/v) of total slurry was used as
substrate. The substrate was added with 10% (v/v) Aspegillus niger (6,5 x 107
CFU/cc) of total slurry and then
inoculated with 10% (v/v) yeast Saccharomyces cereviciae (1,5 x 109 CFU/cc).
The SSF experiments were run for 96 hours and the data were investigated five
time namely 0, 24, 48, 72 and 96 hours. The results showed that alkalinity and
sulfidity effect to yield of pulp, kappa number and lignin content. The best pretreatment to production
bioethanol from oil palm stem was using alkalinity 16% and sulfidity 20% which
produce yield of pulp 39,17%. total of sugar and reducing sugar tended to
decrease with time of inoculation whereas ethanol concentration increase
significantly. The growth of Aspegiluus niger and Saccharomyces cereviciae
yeast tended to incease in initial inoculation and decrease by the end of
inoculation. The best of inoculation time in bioethanol production was 96 hours
which produce bioethanol concentration, yield of bioethanol and bioethanol
productivity were 0,31%; 1,94% and 470,88 litre/ha/year.
Key
words: Bioethanol, pretreatments, oil
palm, kraft process, simulataneous
saccharification and fermentation